Binding of Harmine Derivatives to DNA: A Spectroscopic Investigation

Binding of Harmine Derivatives to DNA: A Spectroscopic Investigation

Abstract

Harmine belongs to a group of β-carboline alkaloids endowed with antitumor properties. Harmine and its derivatives are thought to bind to DNA and interfere with topoisomerase activities. We investigated the base-dependent binding of harmine, and three of its synthetic anticancer-active derivatives to the genomic DNA from calf thymus and two synthetic 20-mer double helices, the poly(dG-dC)·poly(dG-dC) and the poly(dA-dT)·poly(dA-dT), by means of UV-Vis and circular dichroism (CD) spectroscopies. The data show that the DNA binding and stabilising properties of the investigated derivatives are base pair-dependent. These results could be used as a guide to design and develop further bioactive analogues.

Pagano, B., Caterino, M., Filosa, R., & Giancola, C. (2017). Binding of Harmine Derivatives to DNA: A Spectroscopic Investigation. Molecules22(11), 1831. 10.3390/molecules22111831

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By | 2018-08-16T15:27:56+00:00 27 October 2017|Tags: , , , |